Hydrogen peroxide decomposition by potato catalase
Analysis
This is what I will do with my results and how I will record and process them.
This is the table in which I will record my observations.
| Experiment | Substrate the ones (%) | *Oxygen produced in 5 min(ml) | #Time taken(20 ml)(s) | Temperature of solution/degrees C | Rate 1(from*) | Q10 balanced rate 1 | Rate 2(from#) | Q10 balanced rate 2 | Substrate concentration,S |
| 1 | 0.0% | 13 | 0 | 0 | 0 | 0 | (moles per litre) | ||
| 2 | 0.0% | 15 | 0 | 0 | 0 | 0 | 0 | ||
| 3 | 0.0% | We | 0 | 0 | 0 | 0 | 0 | ||
| Average | 0.0% | 14 | 0 | 0 | 0 | 0 | 0 | ||
| 1 2 |
5.0% 5.0% |
17.5 | 0.021 | 0.024 | 0.01345895 | 0.015381657 | 1.47 |
I will then:
- Plot a graph of rate against (average) substrate concentration and compare it with the prediction.
- Calculate the amount of enzyme present and compare this with the prediction.
- Calculate the Michaelis constant (KM) for catalase.
- Decide whether the Q10 Formula is accurate for catalase.
- Plot a graph of rate against concentration with all the repeats and the averages on and determine from the line of best fit which results were anomalous.
- Decide what factor might have caused the anomalies
